Staff Research Associate - 135415

The Department of Anesthesiology employs over 200 academic and staff personnel and has an annual operating budget of $40 million in annualized resources between Practice Plan Revenues, VAMC funding, Hospital and Medical Group Funding, UC Foundation, Research Sources and School of Medicine Funds. There are over 80 clinical faculty, 12 Ph.D, faculty and post docs, 41 residents, 16 clinical fellows, 27 Certified Nurse Anesthetists, 1 Physician Assistant and four bargaining units represented. The Department provides Clinical Anesthesia, Critical Care and Pain Services at Hillcrest, Thornton, Shiley, Children's and the VAMC. The Department operates a Pain Clinic at Perlman. The Department's research programs include bench and clinical research programs located at the VAMC, MTF, CTF, BSB, Hillcrest, Thornton and Sanford Consortium for Regenerative Medicine.

Under the supervision of the Principal Investigator, the incumbent will perform a variety of cellular and molecular techniques and large scale multiomic and biometric analysis. Responsibilities include preparation of RNA for next-generation sequencing, preparing and maintaining primary cell cultures, immunohistochemistry, FACS sorting and confocal imaging. The position requires detail-oriented observations and accurate record keeping. The incumbent will also apply coding skills to organize and analyze large format data that includes facial expressions, voice, language, genomics, transcriptomics, proteomics, and additional capture of data as part of the research program. Responsible for providing the Principal Investigator with a written assessment of experimental outcomes. Participates in the planning of new and existing experiments, by studying available literature, then analyzing, troubleshooting and altering conditions under which determinations are made. Responsible for general laboratory maintenance including organization of lab materials, maintaining laboratory databases, placing supply orders, drafting and maintaining animal protocols and assisting with maintaining laboratory equipment. Willingness to teach and collaborate with PhD and Master level graduate students and visiting scholars in technical studies.

• Theoretical knowledge of a life science as typically acquired by a Bachelor's degree in the field of molecular biology, biochemistry, biotechnology, bioengineering or genetics or an equivalent combination of education and experience.

• Knowledge of sterile techniques and culture media/reagents. Demonstrated ability to perform routine RNA isolation and analysis. Experience with basic protein chemistry techniques.

• Coding skills to integrate and analyze large data sets.

• Ability to maintain supplies and consumables for medium size laboratory and its core facilities including meticulous record keeping of ordered and received materials.

• Experience in maintaining general laboratory equipment (e.g. centrifuges, water baths, etc. and specialized equipment (i.e., OROBOROS, Seahorse, Immunofluorescence, Confocal Microscopy)

• Knowledge of EH&S procedures for laboratory safety.

• Intermediate to advance computer skills using MS Word, Excel, PowerPoint, Graphpad Prism, Imaris, Image email system and a variety of web based programs (Genbank).

• Ability to efficiently manage time and organizational skills. Ability to establish priorities, meet multiple and frequently changing deadlines with flexibility. Detail oriented.

• Strong communication skills (both verbal and written) needed to interact professionally and effectively in the work environment.

• Ability to assist lab personnel, maintain and order supplies, coordinate research projects, train personnel on research techniques and use laboratory equipment.

• Skilled in integrating, cleaning, and harmonizing data from multiple sources to ensure accurate participant tracking and communication.

• Experienced in creating visual dashboards to summarize participant metrics and study operations for staff and stakeholders.

• Ability to work with and manage large data sets to communicate with study participants and staff.

• Employment is subject to a criminal background check.

• Must be willing to work with bio-hazardous materials.

• Must be willing to work occasional weekends or extended hours to meet project deadlines and experiments.

• Must be willing to work with Visiting scholars with minimal English proficiency.